Maximize Info from Minimal Amount of Samples!
Explore Beyond Limits: Minuscule Sample? No Problem!
Insights from Multiplex Assays: Small Samples, Big Impact!
Monitor Multiple Genes and Proteins with Minimal Sample.
Richer Gene and Protein Info from Fewer Cells!
Quantify any Proteins and those "Undetectable".
Visionary Research, Passionate Team, Revolutionizing Cancer Immunotherapy!
Save Sample, Time, & Money!

Technology Advantages

Minimal Amount of Samples 

Many genes and proteins are involved in cellular growth regulation or signal transduction and can serve as targets or stratification markers for new therapeutics. The challenge is how to get sufficient amount of samples such as cells or tissues for examining multiple biomolecules, especially for proteins which traditionally are investigated in a large input amount using LC-MS/MS, Western blot, IHC or flow cytometry. At NANOBIOTEC®, we determine the levels of multiple DNAs, RNAs and proteins, and their expression patterns in minimal amount of samples, as few as 1-100 cells or 1 μl of plasma or serum, using our cutting-edge technologies and tools.

Real-time 

Protein and mRNA expression patterns and levels, including activation or inhibition of signaling pathways, as well as protein posttranslational modifications can change immediately and significantly following surgical resection or animal sacrifice at the end of studies. Many results and read-outs from such samples are often misleading, as the data may not reflect the molecular realities of drug effects as they should be while still in the human or animal body. Using our patented technology and standardized protocols for controlled and rapid tissue processing, we acquire minimal samples in a minimally or non-invasive way from patients or live animals and monitor real-time efficacy of a novel therapies before, during and following drug administration. We thus obtain more reliable and valuable information for gene and protein changes and support rational design of dosing strategies or evaluation of efficacy of novel drugs.

Protein Level Data 

Proteins, the targets of most drugs, are often studied through indirect DNA or RNA surrogates, as many proteins lack cost-effective assays for quantitation due to limitation of sample size. Critical information such as post-transcriptional regulation including mRNA stability and posttranslational modifications is often missed. Common technologies require either high amount of input samples or prohibitively complex expertise and sophisticated instruments (such as LC-MS/MS), or both, for direct protein biomarker discovery and screening efforts. We now offer unique, sensitive and reliable assays for many signaling proteins and disease mediators while requiring minimal amount of samples.

Large Scale Data 

Functional proteomics is the large-scale study of proteins at the functional activity level, such as expression and modification. Studies of complex diseases such as cancer and autoimmune diseases have shown that genetic alterations do not account for all of the causes of the disease.  Changes in protein levels and structure have also been shown to play critical roles in disease development and progression, which are not reflected by genetic changes. In cancers, several genetic and epigenetic changes are often required for development of the disease.  Studying large-scale epigenetic changes such as histone methylation /acetylation, protein phosphorylation or cleavage will greatly aid in understanding the causes and determining effective treatment of cancers and other complex diseases.

Multiplex 

Our validated assays for panels of cytokines, chemes, tumor okinsuppressors, oncoproteins and other signaling molecules can be multiplexed up to 150-plex without losing specificity and sensitivity.

Both Qualitative and Quantitative 

Our assays to test drug effects on protein expression can be both qualitative and quantitative. Unlike ELISA and FACS, our assays measure protein amount after separation based on MW/ size or pI/charge changes (such as phosphorylation). Unlike western blotting which is often inconsistent and is semi-quantitative at best, we quantitate the absolute amount of a protein as low as pM level in minimal amount of samples.

Better Data With Less Animal Use 

Coordinated application of pharmacokinetics (PK) and pharmacodynamics (PD) provides a rational, efficient and informative approach to preclinical and clinical drug development. In clinical studies, core or fine needle aspirate biopsies (FNAB) are used routinely for PK/PD evaluation. In preclinical studies whole tumor samples are often collected for the same studies requiring killing of animals at each time point and hence the use of large numbers of animals. Using our patented micro-biopsy sampling technique combined with sensitive assays, we reduce animal use to determine PK/PD responses and also capture the transient changes of target proteins, as well as make it possible to perform longitudinal studies to evaluate protein changes leading to development of drug resistance without using large numbers of animals.

Diverse Assays and Formats 

We have a panel of cell lines, bioassays and animal models supporting drug discovery and development, from preclinical to clinical studies.

IP and Confidentiality Policy

At NANOBIOTEC®, we understand that protection of our clients' IP and confidential Information is of the utmost importance. We stand by our emphasis on long-term collaborations and meet this challenge by providing comprehensive protective measures. Our strict IP and confidentiality policy is based upon:
  • Preparing our teams with the correct mindset for quality IP protection through rigorous, repeated training.
  • Preventing information leaks in all possible forms.
  • Laboratory notebooks are strictly monitored, regulated, and filed.
  • Ensuring employees' legal compliance with company regulations.
  • Employees are required to sign a CDA agreement prior to employment.


Terms and Conditions

At NANOBIOTEC®, we represent and warrant that all services shall be completed in a professional, workmanlike manner, with the degree of skill and care that is required by current, good and sound professional procedures and practices. For more information, see our Terms and Conditions of Services.