Maximize Info from Minimal Amount of Samples!
Explore Beyond Limits: Minuscule Sample? No Problem!
Insights from Multiplex Assays: Small Samples, Big Impact!
Monitor Multiple Genes and Proteins with Minimal Sample.
Richer Gene and Protein Info from Fewer Cells!
Quantify any Proteins and those "Undetectable".
Visionary Research, Passionate Team, Revolutionizing Cancer Immunotherapy!
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In Vitro Pharmacology & Biology Services

In Vitro Discovery Biology & Translational Medicine  
  • Cytotoxicity and proliferation assays: 3 D cell culture; cell cycle analysis; microtubule and mitosis test
  • Angiogenesis assays: endothelial cell (EC) adhesion, tube formation, aorta ring explant, VEGFR signaling
  • Epigenetics assays: Acetyltransferase, DNA Methyltransferase,  HDAC, Histone Demethylase, Histone Methyltransferase, Bromodomain, PARP screening and profiling  
  • Live cell imaging: adhesion, migration, chemotaxis and wound healing
  • Cell free assays: various kinase, phosphatase, and other enzymatic assays 

  • Simultaneous multiplex analysis mRNA and proteins
  • Reporter gene assays
  • In-cell western blot (ICW) based compound screening assays
  • Traditional western blot, SDS-PAGE and 2-D gel
  • Genome-wide gene array, low density gene array, real-time RT-PCR
  • Biomarker validation and measurement using Simple Western 
  • Characterization of mechanism of action of novel drugs
  • Target identification and validation of drug candidates and novel compounds
  • Analysis of post-translational modification of proteins
  • Drug combination screening for synergistic studies  
  • Development of companion diagnostic methods
  • Screening of anti-aging agents for cosmetics

A wide range of assays and cancer cell collection for compound profiling: 
 
 CANCER CELL PROFILING
 
 Profile
 
 Assay Description
 
 Throughput
Proliferation, growth of >100 lines of human cancer cells, including Leukemia, lymphoma, myeloma, melanoma, and cancers of kidney, colon, lung, prostate, cervix, breast, ovarian, uterine, etc   MTT/MTS assay 

384 or 96-well 
 LDH assay 384 or 96-well 
SRB assay, Almar Blue assay  384 or 96-well 
Luminescent cell viability assay  384 or 96-well 
Apoptosis, Cell cycle  Caspase assay   96-well
Annexin V/PI assay   96-well
Other FACS analysis   96-well
Cytokine induction   Cell viability assay with optimized incubation time   96-well
Drug combination and timepoints  CalcuSyn analysis for Combination Index  96-well 
 Custom  Customer defines assay type 96-well 







           

              Hallmark of Cancer Cells


Multiplex Nucleic Acid Quantification (QuantiGene Plex assay)  


 


Multiplex nucleic acid testing allows multiplexed gene expression analysis (RNA targets) and DNA copy number variation (CNV) analysis, to measure up to 80 genes in a single sample. Here is why to choose our multiplex gene assay over qPCR:

 
 
Mutliplex QuantiGene Assay qPCR assay 
 Sample preparation directly from cell/tissue lysates to avoid biases, sample loss, false results  RNA extraction 
 Compatible samples various samples including cells, tissues, FFPE, whole blood, Paxgene blood, isolated RNA and more  Limited 
RNA purification  NOT needed, to eliminate contamination  Needed 
cDNA synthesis  NOT needed, to eliminate bias  Needed 
 PCR amplication Amplify signals using  branch DNA technology Amplify target genes 
 
Protein and gene expression
 
simultaneous quantification
 
Limited
Multiplex up to 80 genes in a single well without cross-reactivity

Very limited, need multiple attempts; concern of specificity and sensitivity
 Validation Validated panels of targets available Limited
 Customizable Yes Yes
 Z' factor Higher Loweer
Cost 50% lower, more data per sample Higher
Data delievery  Faster, validated gene panels avoiding primer design/optimization Slower







        Branch DNA technology
















 3-D In Vitro Models  

  • 3-D anti-psoriatic drug screening 
  • 3-D cancer cell viability assay                                                                                                            
  • 3-D spheroid cell invasion assay
  • 3-D tumor micro-tissue assay
  • 3-D anti-skin aging cosmetic ingredient screening

Animal Model for Cancer and Inflammation  

  • Mouse tumor models using patient cancer cells or cell lines (PDX or xenograft models) 
  • Rodent inflammation models
  • Angiogenesis models using mice and zebra-fish
  • Micro-biopsy sampling and real-time monitoring efficacy and toxicity of drugs