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Insights from Multiplex Assays: Small Samples, Big Impact!
Monitor Multiple Genes and Proteins with Minimal Sample.
Richer Gene and Protein Info from Fewer Cells!
Quantify any Proteins and those "Undetectable".
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Biomarker & Immunoassay Services

 

Luminex Assay (Beads-based Multiplex Immunoassay)  

  • Quantitation and detection of cytokines / chemokine, cancer biomarkers or signal transduction proteins in cell lysates, cell culture supernatants, serum, plasma, etc
  • >300 human cytokines/chemokines and >100 mouse cytokines can be assayed
  • Simultaneously detect and quantify multiple analytes (proteins and peptides, or nucleic acids) in a single sample volume, with superior performance and reproducible results
  • Simultaneously interrogate multiple protein phosphorylation and histone posttranslational modification
  • Our Bio-Plex® system with high-throughput fluidics is a suspension array system capable of analyzing hundreds of biomolecules in a single sample of small amount such as 25 µl



Simoa Assay (SIngle Molecule Array-based Ultra-sensitive Immunoassay)  

  • A fully automated, ultra-sensitive, multiplexed immunoassay or Digital ELISA.  
  • Quantification of low-abundance biomarkers difficult or impossible to measure with conventional assays. 
  • Quantification of proteins as low as femtogram (fg)/ml in the samples
  • The digital nature allows 1000 x sensitivity increase over conventional ELISA assays with CVs < 10%.

ELISA (Enzyme-Linked Immunosorbent Assay) / ELISpot (Enzyme-Linked ImmunoSpot assay)  

  • We offer ELISA assays in various formats: direct or indirect, standard sandwich or competitive ELISA, as well as ELISpot and In-cell ELISA
  • Our ELISpot detects Ag-specific T cells in frequency as low as 0.0001% within PBMC, i.e., a single cytokine-secreting T cells can be reliably identified among a million bystander cells. 

  • ELISpot is very useful for determining cell mediated immunity due to high sensitivity and specificity in detecting rare Ag-specific cells, providing quantitative information regarding specific cytokine or other secreted immune molecules. 
  • Our ELISpot is frequently used for immunogenicity testing, immune response monitoring, epitope discovery

DELPHIA (Dissociation Enhanced Lanthanide Fluorescent Immunoassay)  

  • Is a heterogeneous, time-resolved fluorescence (TRF) assay that detects reagents labeled with lanthanide chelates (Eu, Tb and Sm)

  • Our DELFIA offers excellent flexibility for a range of applications, when the assay must be highly sensitive, specific, reliable, and reproducible. It is ideal for complex sample matrices and is particularly well suited for TRF, kinase, and immunodetection assays, as well as discovery work.

  • Our DELFIA has a wide dynamic range, is ideal for post-screening applications such as PK, ADA, biomarker and cell-based assay support.

AlphaLisa (Amplified LuminescentProximity Homogeneous Assay)   

  • Virtually any target can be easily measured using AlphaLISA, an improved alternative to quantitative ELISA assays. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messengers, DNA, RNA, proteins, protein-protein interactions, peptides, and small molecules.

  • Our AlphaLISA  is a homogeneous assay with no wash requirement and no separation steps, with a high sensitivity and large dynamic range: detect down to femtogram levels of target
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates and more

RPPA (Reverse Phase Protein Array)  

  • A high-throughput antibody-based micro-array with procedures similar to Western blots
  • A cost-effective, high-efficiency method utilizing automation for increased quality and reliability
  • Proteins are extracted from tissues or cells, denatured by SDS, printed on nitrocellulose-coated slides followed by antibody probe.
  • Simple sample preparation, which is similar to Western blots
  • Robust quantification due to serial dilution and replicates of samples
  • Characterization of cellular signaling networks under different conditions
  • Determination of drug selectivity and Identifying therapeutic targets
  • Defining regulatory mechanisms in signaling networks, including feedback loops and cross-talks
  • Classifying patient tumors,  determining prognosis and predicting responses to targeted therapies
  • Understanding pharmaco-dynamics and biologically relevant dosing and schedule
     
 

Nano-Proteomic Proximity Ligation Assay (PLA)   

  • Quantification of up to 96 proteins in 96 samples simultaneously
  • High grade multiplexing with no trade-off in data quality, saving time and sample by simultaneously analyzing multiple proteins
  • Exceptional specificity - based on the proprietary proximity ligation assay
  • Minimal sample consumption – target proteins can be examined as little as 1 µl of precious samples such as serum, plasma, tissue, etc.
  • High throughput – 9,216 data-points generated per run, discovering accurate information about novel gene expression patterns at the protein level
  • Highly sensitive - unravel low-abundance proteins